Efficacy of 17 α‐methyl testosterone and letrozole on sex reversal of protogynous grouper, Epinephelus tauvina (Forskal, 1775) and spawning performance of sex‐reversed males

Ranjan, Ritesh and Xavier, Biji and Dash, Biswajit and Edward, Loveson and Relangi, Suresh D and Pilli, Suresh Kumar (2015) Efficacy of 17 α‐methyl testosterone and letrozole on sex reversal of protogynous grouper, Epinephelus tauvina (Forskal, 1775) and spawning performance of sex‐reversed males. Aquaculture Research, 46. pp. 2065-2072.

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    Abstract

    This study aimed to test the efficacy of 17 α‐methyl testosterone (17 α‐MT) alone and in combination with letrozole, an aromatase inhibitor, for the induction of sex reversal in protogynous greasy grouper, Epinephelus tauvina. Further, the long‐lasting effects of these treatments and spawning performance of sex‐reversed males were also investigated. Greasy grouper with oocytes in the perinucleolus stage were implanted with 5 mg 17 α‐MT kg−1 body weight (T1), 5 mg 17 α‐MT and 0.2 mg letrozole kg−1 body weight (T2) and 5 mg 17 α‐MT with 0.4 mg letrozole kg−1 body weight (T3) and no androgens/enzyme inhibitor implanted (C). The 17 α‐MT alone and in combination of letrozole‐induced sex reversal in greasy grouper, whereas untreated control fish (C) showed normal ovarian development. However, T2 and T3 group showed 100% sex reversal and completion of spermatogenesis up to functional male phase in 2 and 3 months, respectively, whereas T1 group resulted in only 66.67% functional male with motile spermatozoa after 4 months. Sex‐reversed males successfully fertilized the eggs during induced spawning. There were significant differences on fertilization and hatching rates between T2 group (79.00 ± 4.36%; 77.67 ± 2.87%, respectively) and T1 group (57.67 ± 3.17%; 63.87 ± 2.91%, respectively). The result suggested that 17 α‐MT (5.0 mg kg−1 BW) in combination with letrozole (0.2 mg kg−1 BW) has the potential to produce 100% sex‐reversed male in short period in greasy grouper, which might greatly help in seed production of greasy grouper.

    Item Type: Article
    Uncontrolled Keywords: Grouper, sex reversal, letrozole, 17 a methyl testosterone, spawning
    Subjects: Fish and Fisheries > Biochemical Study
    Fish and Fisheries > Fish breeding
    Fish Biotechnology
    Demersal Fishes > Groupers
    Divisions: CMFRI-Visakhapatnam
    Depositing User: Arun Surendran
    Date Deposited: 20 Feb 2019 08:48
    Last Modified: 23 Feb 2019 10:45
    URI: http://eprints.cmfri.org.in/id/eprint/13406

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