Bhavani, C N (2002) Effect of cryoprotectants on the morphology of fish spermatozoa. Masters thesis, Central Institute of Fisheries Education.
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Abstract
A study was carried out to identify the best cryoprotectant for the cryopreservation of spermatozoa of Liza parsia, a brackish / marine fish of great culture potential. Various cryoprotectants alone and in combinations, at different concentrations have been tried. Morphology of spermatozoa before and after cryopreservation studied by transmission electron microscopy (TEM). A combination of 5% DMSO and 5% glycerol in V2E extender accorded better protection than 10% DMSO alone. The toxic effects of high concentrations of DMSO can be minimised by combining with glycerol.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Cryoprotectants; Morphology; Fish Spermatozoa |
| Subjects: | Dissertations Fish and Fisheries > Fish breeding |
| Divisions: | CMFRI-Kochi > Mariculture Division Subject Area > CMFRI > CMFRI-Kochi > Mariculture Division CMFRI-Kochi > Mariculture Division Subject Area > CMFRI-Kochi > Mariculture Division |
| Depositing User: | Users 17220 not found. |
| Date Deposited: | 05 Sep 2016 09:34 |
| Last Modified: | 08 Apr 2022 08:46 |
| URI: | http://eprints.cmfri.org.in/id/eprint/11037 |
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