Suja, C P and Sukumaran, N and Dharmaraj, S (2007) Effect of culture media and tissue extracts in the mantle explant culture of abalone, Haliotis varia Linnaeus. Aquaculture, 271 (1-4). pp. 516-522.
![[img]](https://eprints.cmfri.org.in/style/images/fileicons/application_pdf.png)
|
PDF
20.pdf Download (153kB) |
Abstract
The study is aimed at developing appropriate media for the mantle explant culture of abalone Haliotis varia. The effect of different media viz., L-15, Ham's F12, M199 was studied in combination with 10% Fetal Calf Serum (FCS) and 10% tissue extracts of gonad, mantle and whole body of abalone, H. varia to understand cell behaviour, cell yield and cell adherence in mantle explant culture of H. varia. Cultures with L-15 media gave better cell yield and M199 promoted better cell adherence. Addition of mantle extract to all media enhanced the cell yield to a maximum followed by whole body extract. Addition of whole body extract facilitated in cell adherence followed by the addition of mantle extract.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Effect of culture media and tissue extracts in the mantle explant culture of abalone, Haliotis varia Linnaeus |
| Subjects: | Molluscan Fisheries |
| Divisions: | CMFRI-Kochi > Marine Capture > Molluscan Fisheries Division Subject Area > CMFRI > CMFRI-Kochi > Marine Capture > Molluscan Fisheries Division CMFRI-Kochi > Marine Capture > Molluscan Fisheries Division Subject Area > CMFRI-Kochi > Marine Capture > Molluscan Fisheries Division |
| Depositing User: | Arun Surendran |
| Date Deposited: | 26 Oct 2010 08:44 |
| Last Modified: | 09 Sep 2015 15:34 |
| URI: | http://eprints.cmfri.org.in/id/eprint/5671 |
Actions (login required)
 |
View Item |
