In vitro efficacy of aquaculture antimicrobials and genetic determinants of resistance in bacterial isolates from tropical aquaculture disease outbreaks

Sumithra, T G and Sharma, S R Krupesha and Suja, G and Nair, V Anusree and Sachidanandan, Suryagayathri and Prasad, Vishnu and Lailaja, V P and George, J C and Patil, Prasanna Kumar (2024) In vitro efficacy of aquaculture antimicrobials and genetic determinants of resistance in bacterial isolates from tropical aquaculture disease outbreaks. Letters in Applied Microbiology, 77 (10). pp. 1-10. ISSN 0266-8254

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Abstract

Understanding the efficacy of antimicrobials against pathogens from clinical samples is critical for their responsible use. The manuscript presents in vitro efficacy and antimicrobial resistance (AMR) genes in seven species of fish pathogens from the disease outbreaks of Indian aquaculture against oxytetracycline, florfenicol, oxolinic acid, and enrofloxacin. In vitro efficacy was evaluated by minimum inhibitory concentration and minimum bactericidal concentration. The gene-specific PCR screened AMR genes against quinolones (qnrA, qnrB, and qnrS) and tetracyclines (tetM, tetS, tetA, tetC, tetB, tetD, tetE, tetH, tetJ, tetG, and tetY). The results showed that Aeromonas veronii (45%) showed the maximum resistance phenotype, followed by Streptococcus agalactiae (40%), Photobacterium damselae (15%), Vibrio parahaemolyticus (10%), and Vibrio vulnificus (5%). There was no resistance among Vibrio harveyi and Vibrio alginolyticus against the tested antimicrobials. The positive association between tetA, tetB, tetC, tetM, or a combination of these genes to oxytetracycline resistance and qnrS to quinolone resistance indicated their potential in surveillance studies. The prevalence of resistance phenotypes (16.43%) and evaluated AMR genes (2.65%) against aquaculture antimicrobials was low. The resistance phenotype pattern abundance was 0.143. All the isolates showed susceptibility to florfenicol. The results help with the appropriate drug selection against each species in aquaculture practices.

Item Type: Article
Uncontrolled Keywords: antimicrobial resistance; Vibrio; Aeromonas veronii; Streptococcus agalactiae; antimicrobial resistance genes
Subjects: Aquaculture > Tropical fish
Biochemistry
Fish and Fisheries > Fish Disease
Fish and Fisheries > Fish Genetics
Fish and Fisheries > Fish Pathology
Fish Biotechnology
Divisions: CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division
Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division
CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division
Subject Area > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division
Depositing User: Arun Surendran
Date Deposited: 24 Oct 2024 10:50
Last Modified: 24 Oct 2024 10:50
URI: http://eprints.cmfri.org.in/id/eprint/18974

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