Different methods for microalgae isolation

Ranjan, Ritesh and Xavier, Biji and Sadhu, Narasimhulu and Bathina, Chinnibabu (2024) Different methods for microalgae isolation. In: Marine Microalgae culture Techniques. CMFRI Training Manual Series No. 46/2024 . ICAR-Central Marine Fisheries Research Institute, Visakhapatnam, pp. 17-23.

Text Marine Microalgae culture Techniques_2024_Ritesh Ranjan.pdf Download (1MB)

Abstract

Once the decision of which microalgae needs to be cultured is taken, the first step is to isolate the pure culture. Even though the cultures are available from specialized culture collection centers, isolation of endemic strain is advisable because the strain can grow better in local environmental conditions. Collection of micro algal sample is the crucial step which can be done using plankton nets of mesh size 10, 30 and 120 µm. The collected micro algae need to be kept in sterile nutrient solution to keep them alive and allow them to grow. Some amount of the sample can be concentrated and preserved in 10% buffered formalin for the identification in the laboratory. While sample collection both biotic (light, water temperature, dissolved oxygen, dissolved carbon dioxide, nutrient concentration, pH, salinity) and abiotic factors (pathogen) at the sampling site need to be considered. Technique used for the isolation of micro algae includes technique of dilution, single cell isolation by micropipette and agar streaking.

Actions (login required)

View Item