The principles of isolation, purification and analysis of nucleic acids

Paulton, M P and Thomas, P C (2003) The principles of isolation, purification and analysis of nucleic acids. In: Winter school on Recent Advances in Mariculture Genetics and Biotechnology, 4- 24 November 2003, Cochin.

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Abstract

Advanced Biotechnological research is largely depended on the genome analysis and recombinant DNA technology. Good quality nucleic acid is an essential prerequisite for consistent results in most of the down stream applications in the genome analysis and recombinant DNA technology. The general principle underlying the isolation of nucleic acids is common with few modifications depending on the type of nucleic acids being isolated. The type of the nucleic acid intending to isolate is to be made free from the other biological macromolecules and cell debris. This is achieved by properly lysing the cell wall or cell membrane as the case may be and by selectively denaturing the other macromolecules like proteins.

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