Characterization of haemocytes of the Indian edible oyster, Crassostrea madrasensis (Preston)

Ittoop, Gijo and George, K C and Sanil, N K and George, Rani Mary and Sobhana, K S and Nisha, P C (2006) Characterization of haemocytes of the Indian edible oyster, Crassostrea madrasensis (Preston). Aquaculture Research, 37. pp. 1636-1643.

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Abstract

The haemocytes of the Indian edible oyster Crassostrea madrasensis were characterized using light and electron microscopy. The light microscopic study was conducted by staining a monolayer of the haemocytes with Geimsa. Cells without granules and with a large nucleus occupying much of the cytoplasmic area were grouped as hyalinocytes. Those with lesser amounts of basophilic cytoplasmic granules were characterized as semigranulocytes and those with large amounts of a mixture of acidophilic and basophilic granules were termed as granulocytes. Ultrastructural studies also revealed the presence of three types of haemocytes. Scanning electron microscopic studies were used to study the spreading behaviour of the haemocytes. Cytochemical studies revealed the presence of acidphosphatase, peroxidase and prophenol oxidase in the cells.

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