Manual of techniques for estimating bacterial growth rates, productivity and numbers in aquaculture ponds

Moriarty, D J W and Chandrika, V (1987) Manual of techniques for estimating bacterial growth rates, productivity and numbers in aquaculture ponds. CMFRI Special Publication, 42. pp. 1-27.

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Abstract

Heterotrophic microorganisms, especially bacteria, play an important part in decomposition processes, nutrient cycling and food chains in aquatic systems. A number of different methods have been proposed for measuring microbial growth rates, but many are not specific for bacteria or do not include the whole population. It is evident that the ideal method should involve minimal handling of the bacterial population and be applied quickly so as not to alter natural or in situ growth rates or to be influenced by bacterial grazers. The use of radioactive nucleicacid precursors especially thymidine, to measure the rate of DNA synthesis has many of the prerequisites of the ideal method. As with all other techniques, there are disadvantages as well as advantages in using measurements of nucleicacid synthesis

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