Diwan, A D and Joseph, Shoji (1999) Cryopreservation of spermatophores of the marine shrimp, Penaeus indicus H. Milne Edwards. Indian Journal of Fisheries, 46 (2). pp. 159-166.
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Abstract
Attempts on cryopreservation of spermatozoa have been made in penaeid shrimp, Penaeus indicus. Viable spermatozoa could be preserved successfully for a period of 60 days at -35°C and -196°C. Among several cryoprotectants used, a combination of DMSO (5 %) and glycerol (5 %), DMSO (5 %) and trehalose (0.25M) showed best viability rate (75 to 80 %) in freeze, thawed spermatozoa. Activational changes in spermatozoa during acrosome reaction after induction with egg water have also been recorded. The importance of cryogenic storage of spermatozoa is discussed.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Marine shrimp; Penaeus indicus; Cryoperservation; spermatophores |
| Subjects: | Fish and Fisheries > Fish physiology Crustacean Fisheries > Prawn and Prawn fisheries |
| Divisions: | CMFRI-Kochi > Physiology and Nutrition Pathology Subject Area > CMFRI > CMFRI-Kochi > Physiology and Nutrition Pathology CMFRI-Kochi > Physiology and Nutrition Pathology Subject Area > CMFRI-Kochi > Physiology and Nutrition Pathology |
| Depositing User: | Dr. V Mohan |
| Date Deposited: | 16 Mar 2010 05:49 |
| Last Modified: | 09 Sep 2015 15:05 |
| URI: | http://eprints.cmfri.org.in/id/eprint/160 |
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