Marine-derived polygalactofucan and its β-2-deoxy-aminosubstituted glucopyranan composite attenuate 3-hydroxy-3- methylglutaryl-CoA reductase: prospective natural anti-dyslipidemic leads

Chakraborty, Kajal and Anusree, M (2020) Marine-derived polygalactofucan and its β-2-deoxy-aminosubstituted glucopyranan composite attenuate 3-hydroxy-3- methylglutaryl-CoA reductase: prospective natural anti-dyslipidemic leads. Medicinal Chemistry Research, 29 (2). pp. 281-300.

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Abstract

Adverse side effects reported for the use of statin drugs provided insights to develop potential anti-dyslipidemic derivatives from natural origin. The objective of the work was to develop the marine-derived polysaccharides attenuating 3-hydroxy-3- methylglutaryl-CoA reductase (HMGCR), and as prospective natural anti-dyslipidemic leads. Physical and chromatographic purification methods were used to isolate the polygalactofucan from the marine macroalga Sargassum wightii and β-(2- deoxy)-amino-substituted glucopyrananan from a marine crustacean. Glycosidic linkage analysis by the process of methylation was used for structural elucidation of the polygalactofucan, and the methylated and partially methylated alditol acetates were characterized using extensive spectroscopic experiments. The polysaccharide composite constituting the titled polysaccharide motifs showed significant HMGCR inhibitory potential (IC90 0.12 mg mL−1) and an increase in HMG-CoA/ mevalonate ratio (1.68 mg dL−1) compared with the high-fat diet (HFD)-treated animals (1.04 mg dL−1), which recognized its hypo-lipidemic efficacy. In vivo results demonstrated about 70% reduction in the triglyceride levels with the concomitant increase (~39%) of hepatic lipoprotein lipase (LPL) activity in the HFD-fed Wistar rats treated with 500 mg kg−1 body weight. The results illustrated the use of marine-derived polygalactofucan composite as potential anti-dyslipidemic agent.

Item Type:	Article
Uncontrolled Keywords:	Anti-dyslipidemic agent; Polygalactofucan composite; β-(2-deoxy)-amino-substituted glucopyrananan; Partially methylated alditol acetates; 3-hydroxy-3-methylglutaryl-CoA reductase; Hypo-lipidemic efficacy
Subjects:	Fish Biotechnology > Bioactive compound Biochemistry > Bioprospecting Biochemistry
Divisions:	CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division Subject Area > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division
Depositing User:	Arun Surendran
Date Deposited:	24 Jan 2020 04:38
Last Modified:	24 Jan 2020 04:38
URI:	http://eprints.cmfri.org.in/id/eprint/14049

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