Rajesh, K M and Mukherjee, S C and Ranjan, Ritesh and Vani, T and Brahmachari, Rajeev K and Nayak, Sukanta K (2015) Effect of dietary supplementation of Bacillus subtilis on haematological and immunological parameters of Catla catla (Hamilton). Aquaculture International, 23 (5). pp. 1275-1292.
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Abstract
Effect of dietary supplementation of a Gram-positive, aerobic, probiotic bacterium Bacillus subtilis on the immunohaematological indices during pre- and post-challenge in Indian major carp, catla (Catla catla), was studied. The B. subtilis was administered orally at four different doses 1.0 × 106 (T1), 1.0 × 107 (T2), 1.0 × 108 (T3), and 1.0 × 109 (T4) cfu g−1 feed to C. catla for 90 days. The positive control (Cp) and negative control (Cn) were fed with feed without B. subtilis for the same period. On the 60th day, blood and serum were sampled to determine various haematological and serum parameters. Fish were challenged intraperitoneally with Aeromonas hydrophila after 60 days in all the treatment groups and Cp, while the Cn was challenged with phosphate-buffered saline (PBS, pH 7.2) only. Dietary supplementation of B. subtilis leads to the rise of various immunological and haematological parameters in catla during the pre- and post-challenge. During pre-challenge, the highest TEC (1.30 ± 0.02 × 106 cells mm−3), haemoglobin (7.43 ± 0.25 g %), total serum protein (3.89 ± 0.08 g dL−1), and serum lysozyme activity (8.39 ± 0.01 µg ml−1) were recorded in fishes fed feed containing B. subtilis at 1 × 109 cfu/g feed (T4). The highest survival percentage (86.33 %) was also observed in T4 group. The significantly increased survival percentage (P < 0.05) of B. subtilis-treated groups in comparison with control group (Cp) suggests that dietary supplementation of this probiotic bacterium can protect catla from A. hydrophila infection by enhancing innate immunity.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Bacillus subtilis; Aeromonas hydrophila; Catla catla; Immunity; Respiratory burst activity; Total erythrocyte count |
| Subjects: | Fish and Fisheries > Fish Pathology Fish Biotechnology |
| Divisions: | CMFRI-Visakhapatnam |
| Depositing User: | Arun Surendran |
| Date Deposited: | 20 Jan 2020 09:29 |
| Last Modified: | 20 Jan 2020 09:29 |
| URI: | http://eprints.cmfri.org.in/id/eprint/14043 |
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