DNA barcoding Indian marine fishes

Lakra, W S and Verma, M S and Goswami, Mukunda and Lal, Kuldeep Kumar and Mohindra, Vindhya and Punia, P and Gopalakrishnan, A and Singh, K V and Ward, Robert D and Hebert, P (2011) DNA barcoding Indian marine fishes. Molecular Ecology Resources, 11. pp. 60-71.

Preview

Text AGKN_2011_Molecular Ecology Resources_DNA barcoding Indian marine.pdf Download (352kB) | Preview

Abstract

DNA barcoding has been adopted as a global bio-identification system for animals in recent years. A major national programme on DNA barcoding of fish and marine life was initiated in India by the authors during 2006 and 115 species of marine fish covering Carangids, Clupeids, Scombrids, Groupers, Sciaenids, Silverbellies, Mullids, Polynemids and Silurids representing 79 Genera and 37 Families from the Indian Ocean have been barcoded for the first time using cytochrome c oxidase I gene (COI) of the mtDNA. The species were represented by multiple specimens and a total of 397 sequences were generated. After amplification and sequencing of 707 base pair fragment of COI, primers were trimmed which invariably generated a 655 base pair barcode sequence. The average Kimura two parameter (K2P) distances within species, genera, families, orders were 0.30%, 6.60%, 9.91%, 16.00%, respectively. In addition to barcode-based species identification system, phylogenetic relationships among the species have also been attempted. The neighbour-joining tree revealed distinct clusters in concurrence with the taxonomic status of the species.

Actions (login required)

View Item