Cryopreservation of spermatophores of the marine shrimp Penaeus indicus H. Milne Edwards

Diwan, A D and Joseph, Shoji (1998) Cryopreservation of spermatophores of the marine shrimp Penaeus indicus H. Milne Edwards. In: Fish Genetics and Biodiversity Conservation. Ponniah, A G and Das, P and Verma, S R,(eds.) Nature Conservators, Mussafarnagar, pp. 243-251.

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Abstract

Attempts on cryopreservation of spermatozoa have been made in penaeid shrimp Penaeus indicus. It was observed that viable spermatozoa could bepreserved successfully for a period of60 days at -35"C and -196"C temperature. Among several cryoprotectantsused a combination of DMSO (5%) + glycerol (5%) and DMSO (5%) + trehalose (0.25 M) showed best viability rate (75 to 80%) in freeze thawed spermatoza. The activational changes in spermatozoa during acrosome reaction after induction with anegg water have also been mentioned. The importance of cryogenic storage of spermatozoa has been discussed.

Item Type:Book Section
Uncontrolled Keywords:Cryopreservation; spermatophores; marine shrimp; Penaeus indicus
Subjects:Crustacean Fisheries > Prawn and Prawn fisheries
Divisions:CMFRI-Cochin > Marine Capture > Crustacean Fisheries
ID Code:7896
Deposited By:Arun Surendran
Deposited On:05 Mar 2011 17:35
Last Modified:26 May 2011 13:43

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