Diwan, A D and Joseph, Shoji (1998) Cryopreservation of spermatophores of the marine shrimp Penaeus indicus H. Milne Edwards. In: Fish Genetics and Biodiversity Conservation. Ponniah, A G and Das, P and Verma, S R,(eds.) Nature Conservators, Mussafarnagar, pp. 243-251.
Attempts on cryopreservation of spermatozoa have been made in penaeid shrimp Penaeus indicus. It was observed that viable spermatozoa could bepreserved successfully for a period of60 days at -35"C and -196"C temperature. Among several cryoprotectantsused a combination of DMSO (5%) + glycerol (5%) and DMSO (5%) + trehalose (0.25 M) showed best viability rate (75 to 80%) in freeze thawed spermatoza. The activational changes in spermatozoa during acrosome reaction after induction with anegg water have also been mentioned. The importance of cryogenic storage of spermatozoa has been discussed.
|Item Type:||Book Section|
|Uncontrolled Keywords:||Cryopreservation; spermatophores; marine shrimp; Penaeus indicus|
|Subjects:||Crustacean Fisheries > Prawn and Prawn fisheries|
|Divisions:||CMFRI-Cochin > Marine Capture > Crustacean Fisheries|
|Deposited By:||Arun Surendran|
|Deposited On:||05 Mar 2011 17:35|
|Last Modified:||26 May 2011 13:43|
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