Cryopreservation of spermatophores of the marine shrimp Penaeus indicus H. Milne Edwards

Diwan, A D and Joseph, Shoji (1998) Cryopreservation of spermatophores of the marine shrimp Penaeus indicus H. Milne Edwards. In: Fish Genetics and Biodiversity Conservation. Nature Conservators, Mussafarnagar, pp. 243-251.

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    Abstract

    Attempts on cryopreservation of spermatozoa have been made in penaeid shrimp Penaeus indicus. It was observed that viable spermatozoa could bepreserved successfully for a period of60 days at -35"C and -196"C temperature. Among several cryoprotectantsused a combination of DMSO (5%) + glycerol (5%) and DMSO (5%) + trehalose (0.25 M) showed best viability rate (75 to 80%) in freeze thawed spermatoza. The activational changes in spermatozoa during acrosome reaction after induction with anegg water have also been mentioned. The importance of cryogenic storage of spermatozoa has been discussed.

    Item Type: Book Section
    Uncontrolled Keywords: Cryopreservation; spermatophores; marine shrimp; Penaeus indicus
    Subjects: Crustacean Fisheries > Prawn and Prawn fisheries
    Divisions: CMFRI-Kochi > Marine Capture > Crustacean Fisheries
    Subject Area > CMFRI Brochures > CMFRI-Kochi > Marine Capture > Crustacean Fisheries
    CMFRI-Kochi > Marine Capture > Crustacean Fisheries
    Depositing User: Arun Surendran
    Date Deposited: 05 Mar 2011 12:05
    Last Modified: 09 Sep 2015 15:46
    URI: http://eprints.cmfri.org.in/id/eprint/7896

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