Laboratory - scale high density culture of the marine diatom Chaetoceros sp.

Lambade, S B and Mohamed, K S (2002) Laboratory - scale high density culture of the marine diatom Chaetoceros sp. Indian Journal of Fisheries, 49 (1). pp. 13-22.



A laboratory scale continuous and semi-continuous system for culture of the marine diatom Chaetoceros sp. was devised. Using 2.5 times the normal nutrient concentration and limited supply of Co2, the culture density could be increased from an average 1.5 million cells / ml concentration in traditional batch culture systems to 13.7 million cells/ml in continuous system. Besides the increased biomass production, the duration of culture was enhanced to thirty days without crashing. The semi-continuous system was up-scaled to 60 1 capacity with an internal illumination system to yield 528 litres of average 3.2 million cells/ml within 36 days. The velocity of cell growth during the logarithmic phase in doublings/day ranged from 0.21 in control (batch culture) to 2.51 in the semi-continuous system. Furthermore, the cost of production, even with additional inputs, are comparable or even less than the traditional batch cultures. The economic efficiency was highest in continuous systems as compared to semi-continuous systems (Rs. 0.03 and 0.017/billion cells as against Rs. 0.082 and 0.037/billion cells).

Item Type:Article
Official URL/DOI:
Uncontrolled Keywords:Marine diatom; Chaetoceros sp.
Divisions:CMFRI-Cochin > Marine Capture > Molluscan Fisheries
ID Code:70
Deposited By:Dr. V Mohan
Deposited On:10 Mar 2010 12:53
Last Modified:10 Mar 2010 12:53

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