Biochemical composition of the ovary and partial characterisation of yolk protein vitellin in Metapenaeus monoceros (Fabricius, 1798)

Abraham, Joice and Manisseri, Mary K (2007) Biochemical composition of the ovary and partial characterisation of yolk protein vitellin in Metapenaeus monoceros (Fabricius, 1798). Indian Journal of Fisheries, 54 (3). pp. 299-304.

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    Abstract

    Yolk protein vitellin in ‘speckled shrimp’ Metapenaeus monoceros was partially characterised employing polyacrylamide gel electrophoresis (PAGE). There was an increase in the level of expression of vitellin in the ovary, with the advancement of maturation. The vitellin fraction was not expressed in immature and spent ovaries. Molecular weight of vitellin was 326 kDa. Confirmation of the vitellin band by selective staining showed that the yolk protein was a lipoglyco- carotenoprotein possessing a calcium moiety. A protein fraction with molecular weight 270 kDa, which was a lipoprotein, was expressed in ovaries of all maturity stages as well as in testis. The major biochemical constituents of mature yolk of M. monoceros on dry weight basis were protein (59.36±1.18%), lipid (31.23±2.98%) and carbohydrates (2.97±0.81%). Carotenoids accounted for 0.70±0.81% wet weight of yolk.

    Item Type: Article
    Uncontrolled Keywords: Biochemical composition; ovary; yolk protein; vitellin; Metapenaeus monoceros
    Subjects: Biochemistry > Biochemical composition
    Divisions: CMFRI-Kochi > Marine Capture > Crustacean Fisheries
    Subject Area > CMFRI Brochures > CMFRI-Kochi > Marine Capture > Crustacean Fisheries
    CMFRI-Kochi > Marine Capture > Crustacean Fisheries
    Depositing User: Users 171 not found.
    Date Deposited: 30 Oct 2010 10:34
    Last Modified: 09 Sep 2015 15:35
    URI: http://eprints.cmfri.org.in/id/eprint/5866

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