Inhibitory compound produced by Pseudomonas with effectiveness on Vibrio harveyi

Preetha, R and Jose, Seena and Sreedharan, P and Vijayan, K K and Jayaprakash, N S and Philip, Rosamma and Bright Singh, I S (2010) Inhibitory compound produced by Pseudomonas with effectiveness on Vibrio harveyi. Aquaculture Research, 41. pp. 1452-1461.

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    Abstract

    Persistence of the antivibrio property of the potential antagonistic probiotics, Pseudomonas MCCB 102 and 103, at di¡erent temperatures, pH and in organic solvents was studied. The antivibrio compound was extracted, puri¢ed and characterized using thin-layer chromatography, high-pressure liquid chromatography, liquid chromatography-mass spectroscopy, UV^ Vis and nuclear magnetic resonance spectroscopy and identi¢ed as N-methyl-1-hydroxyphenazine, a phenazine antibiotic. The toxicity of the compound was tested in Penaeus monodon haemocyte culture and the IC50 valuewas found to be1.4 _ 0.31mg L_1. The compound was found to be bacteriostatic at 0.5mg L_1. Its stability to varying temperature, pH, organic solvents, prolonged shelf-life and vibriostatic nature point to its suitability for prophylatic aquaculture application.

    Item Type: Article
    Uncontrolled Keywords: antivibrio compound; N-methyl-1- hydroxyphenazine; liquid chromatography-mass spectroscopy; nuclear magnetic resonance spectroscopy; haemocyte culture; aquaculture
    Subjects: Biochemistry > Bioactive compounds
    Fish and Fisheries > Biochemical Study
    Divisions: CMFRI-Kochi > Physiology and Nutrition Pathology
    Subject Area > CMFRI Brochures > CMFRI-Kochi > Physiology and Nutrition Pathology
    CMFRI-Kochi > Physiology and Nutrition Pathology
    Depositing User: Arun Surendran
    Date Deposited: 28 Oct 2010 08:44
    Last Modified: 09 Sep 2015 15:34
    URI: http://eprints.cmfri.org.in/id/eprint/5809

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