Inhibitory compound produced by Pseudomonas with effectiveness on Vibrio harveyi

Preetha, R and Jose, Seena and Sreedharan, P and Vijayan, K K and Jayaprakash, N S and Philip, Rosamma and Bright Singh, I S (2010) Inhibitory compound produced by Pseudomonas with effectiveness on Vibrio harveyi. Aquaculture Research, 41 . pp. 1452-1461.

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Abstract

Persistence of the antivibrio property of the potential antagonistic probiotics, Pseudomonas MCCB 102 and 103, at di¡erent temperatures, pH and in organic solvents was studied. The antivibrio compound was extracted, puri¢ed and characterized using thin-layer chromatography, high-pressure liquid chromatography, liquid chromatography-mass spectroscopy, UV^ Vis and nuclear magnetic resonance spectroscopy and identi¢ed as N-methyl-1-hydroxyphenazine, a phenazine antibiotic. The toxicity of the compound was tested in Penaeus monodon haemocyte culture and the IC50 valuewas found to be1.4 _ 0.31mg L_1. The compound was found to be bacteriostatic at 0.5mg L_1. Its stability to varying temperature, pH, organic solvents, prolonged shelf-life and vibriostatic nature point to its suitability for prophylatic aquaculture application.

Item Type:Article
Uncontrolled Keywords:antivibrio compound; N-methyl-1- hydroxyphenazine; liquid chromatography-mass spectroscopy; nuclear magnetic resonance spectroscopy; haemocyte culture; aquaculture
Subjects:Biochemistry > Bioactive compounds
Fish and Fisheries > Biochemical Study
Divisions:CMFRI-Cochin > Physiology and Nutrition Pathology
ID Code:5809
Deposited By:Arun Surendran
Deposited On:28 Oct 2010 14:14
Last Modified:25 May 2012 13:41

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