Effect of culture media and tissue extracts in the mantle explant culture of abalone, Haliotis varia Linnaeus

Suja, C P and Sukumaran, N and Dharmaraj, S (2007) Effect of culture media and tissue extracts in the mantle explant culture of abalone, Haliotis varia Linnaeus. Aquaculture, 271 (1-4). pp. 516-522.

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Abstract

The study is aimed at developing appropriate media for the mantle explant culture of abalone Haliotis varia. The effect of different media viz., L-15, Ham's F12, M199 was studied in combination with 10% Fetal Calf Serum (FCS) and 10% tissue extracts of gonad, mantle and whole body of abalone, H. varia to understand cell behaviour, cell yield and cell adherence in mantle explant culture of H. varia. Cultures with L-15 media gave better cell yield and M199 promoted better cell adherence. Addition of mantle extract to all media enhanced the cell yield to a maximum followed by whole body extract. Addition of whole body extract facilitated in cell adherence followed by the addition of mantle extract.

Item Type:Article
Official URL/DOI:http://www.elsevier.com/locate/aqua-online
Uncontrolled Keywords:Effect of culture media and tissue extracts in the mantle explant culture of abalone, Haliotis varia Linnaeus
Subjects:Molluscan Fisheries
Divisions:CMFRI-Cochin > Marine Capture > Molluscan Fisheries
ID Code:5671
Deposited By:Arun Surendran
Deposited On:26 Oct 2010 14:14
Last Modified:28 Oct 2010 15:50

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