Biotechnological approach in in-vitro pearl production

Dharmaraj, S and Suja, C P (2001) Biotechnological approach in in-vitro pearl production. In: Perspectives in Mariculture. Menon, N G and Pillai, P P,(eds.) The Marine Biological Association of India, Cochin, pp. 405-412.



Explant culture of mantle tissue of the pearl oyster Pinctada fucata (Gould) was undertaken at the tissue culture laboratory of CMFRI, Tuticorin. Mantle tissues were routinely cultured in Medium 199 and Pf 35 individually and combindly supplemented with 10% foetal calf serum at 28°C. pH 7.8. Explants released numerous epitheloid – like and fibroblast - like cells. Cells were in assorted sizes and formed colonies. Nacreous secretion by the cells was observed on culture plates. The study represents a new tool for cellular approach in in-vitro pearl production.

Item Type:Book Section
Uncontrolled Keywords:Biotechnological approach; in-vitro pearl production
Subjects:Aquaculture > Mariculture
Molluscan Fisheries > Pearl oyster
Divisions:CMFRI-Cochin > Marine Capture > Molluscan Fisheries
ID Code:2461
Deposited By:Geetha P Mrs
Deposited On:19 Aug 2010 14:22
Last Modified:19 Aug 2010 14:22

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