Amylase and acid protease production by solid state fermentation using Aspergillus niger from mangrove swamp

Bhatnagar, D and Imelda, Joseph and Paulraj, R (2010) Amylase and acid protease production by solid state fermentation using Aspergillus niger from mangrove swamp. Indian Journal of Fisheries , 57 (1). pp. 45-51.

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Abstract

Production of amylase and acid protease by solid state fermentation employing Aspergillus niger strain S14, a mangrove isolate was evaluated. Wheat bran containing 60% moisture was used for fermentation with two inoculum sizes of 2x106 and 40x106 spores 10 g substrate-1. The maximum amylase activity of 48.13 Ugdfs-1 was recorded after an incubation period of 4 days at temperature 30 0C and pH 4.8 for the inoculum size 40x106 spores 10 g substrate-1. The concentration of reducing sugar showed a significant negative correlation (-0.496, p<0.05) with amylase production. The peak acid protease activity was observed on day 6 (54.89 Ugdfs-1) for 2x106 spores 10g substrate-1 and on day 4 for 40x106 spores 10g substrate-1 under standard assay conditions of 30 0C and pH 2.7. The concentration of protein in the substrate was significantly (R=0.778, p<0.05) correlated with the acid protease production.

Item Type:Article
Uncontrolled Keywords:Acid protease; Amylases; Aspergillus niger; Mangrove; Solid state fermentation; Wheat bran
Subjects:Fish and Fisheries > Fish physiology
Divisions:CMFRI-Cochin > Physiology and Nutrition Pathology
ID Code:2316
Deposited By:Dr. V Mohan
Deposited On:11 Aug 2010 11:31
Last Modified:03 Nov 2010 11:39

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