Amylase and acid protease production by solid state fermentation using Aspergillus niger from mangrove swamp

Bhatnagar, D and Imelda, Joseph and Paulraj, R (2010) Amylase and acid protease production by solid state fermentation using Aspergillus niger from mangrove swamp. Indian Journal of Fisheries, 57 (1). pp. 45-51.

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    Abstract

    Production of amylase and acid protease by solid state fermentation employing Aspergillus niger strain S14, a mangrove isolate was evaluated. Wheat bran containing 60% moisture was used for fermentation with two inoculum sizes of 2x106 and 40x106 spores 10 g substrate-1. The maximum amylase activity of 48.13 Ugdfs-1 was recorded after an incubation period of 4 days at temperature 30 0C and pH 4.8 for the inoculum size 40x106 spores 10 g substrate-1. The concentration of reducing sugar showed a significant negative correlation (-0.496, p<0.05) with amylase production. The peak acid protease activity was observed on day 6 (54.89 Ugdfs-1) for 2x106 spores 10g substrate-1 and on day 4 for 40x106 spores 10g substrate-1 under standard assay conditions of 30 0C and pH 2.7. The concentration of protein in the substrate was significantly (R=0.778, p<0.05) correlated with the acid protease production.

    Item Type: Article
    Uncontrolled Keywords: Acid protease; Amylases; Aspergillus niger; Mangrove; Solid state fermentation; Wheat bran
    Subjects: Fish and Fisheries > Fish physiology
    Divisions: CMFRI-Kochi > Physiology and Nutrition Pathology
    Subject Area > CMFRI Brochures > CMFRI-Kochi > Physiology and Nutrition Pathology
    CMFRI-Kochi > Physiology and Nutrition Pathology
    Depositing User: Dr. V Mohan
    Date Deposited: 11 Aug 2010 06:01
    Last Modified: 09 Sep 2015 15:16
    URI: http://eprints.cmfri.org.in/id/eprint/2316

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