Cryopreservation of spermatozoa in the mullet, Liza parsia: Effect of cryoprotectants on morphology and motility

Bhavani, C N and Noble, D and Gopalakrishnan, A and Sanil, N K (2003) Cryopreservation of spermatozoa in the mullet, Liza parsia: Effect of cryoprotectants on morphology and motility. Journal of the Marine Biological Association of India, 45 (1). pp. 38-46.

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Abstract

A study was conducted to identify the best cryoprotectant for the spermatozoa of the mullet, Liza parsia (Hamilton-Buchanan), during cryopreservation, based on changes in ultrastructure and motility. Various cryoprotectants alone and in combinations, at different concentrations have been tried. Morphology of spermatozoa before and after cryopreservation was studied by transmission electron microscopy (TEM). A combination of 5% DMSO and 5% glycerol in V2E extender accorded better protection than 10% DMSO alone.

Item Type:Article
Uncontrolled Keywords:Cryopreservation; Spermatozoa; Mullet; Liza parsia
Subjects:Fish and Fisheries > Fish Genetics
Demersal Fishes > Mullet
Divisions:CMFRI-Cochin > Marine Capture > Demersal Fisheries
ID Code:2001
Deposited By:Edwin Joseph
Deposited On:10 Aug 2010 16:28
Last Modified:10 Aug 2010 16:29

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