Development of a novel multiplex-PCR technology for simultaneous detection of five major aquaculture pathogens

Nair, V Anusree and Sumithra, T G and Reshma, K J and Sanil, N K (2023) Development of a novel multiplex-PCR technology for simultaneous detection of five major aquaculture pathogens. Indian Journal of Experimental Biology, 61 (5). pp. 379-388.

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Abstract

Early and precise pathogen identification and corresponding disease management are primary concerns in aquaculture. Here, we attempted at diagnostic methods that can simultaneously identify multiple pathogens, where many samples, several pathogens, and concurrent infections are to be handled. Hence, a multiplex PCR assay targeting five major aquaculture pathogens, viz. Vibrio parahaemolyticus, Vibrio anguillarum, Vibrio alginolyticus, Vibrio vulnificus and Vibrio harveyi was developed for the first time. The primers targeting toxR of V. parahaemolyticus, amiB of V. anguillarum, col of

Item Type:	Article
Uncontrolled Keywords:	Internal amplification control; Vibrio spp.
Subjects:	Fish and Fisheries > Biochemical Study Fish and Fisheries > Fish Disease Fish and Fisheries > Fish Pathology Aquaculture Fish Biotechnology
Divisions:	CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division Subject Area > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division
Depositing User:	Arun Surendran
Date Deposited:	14 Jul 2023 06:25
Last Modified:	14 Jul 2023 06:25
URI:	http://eprints.cmfri.org.in/id/eprint/17239

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