Toxicity assessment of Tetrodotoxin of commercially exploited smooth blassop Lagocephalus inermis in South-Eastern Arabian Sea

Saha, Purbali and Thomas, Sujitha and Badanthadka, Murali and Sharma, S R Krupesha and Mathias, Michelle (2023) Toxicity assessment of Tetrodotoxin of commercially exploited smooth blassop Lagocephalus inermis in South-Eastern Arabian Sea. Regional Studies in Marine Science, 64. pp. 1-10. ISSN 2352 4855

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Abstract

The toxicity assessment of Lagocephalus inermis, commonly known as smooth blassop, which is commercially exploited in the south-eastern Arabian Sea, was conducted in this study, marking the first of its kind for the region. The experiment involved testing control and standard samples during pre-monsoon and post-monsoon seasons. Gross anatomical features were observed, followed by histopathological examination of hearts, livers, and kidneys of deceased mice to determine toxicity. High-performance liquid chromatography (HPLC) was used to analyze standard tetrodotoxin (TTX) concentrations, which showed characteristic peaks at retention times of 4.83 min (mins), 4.792 min, 4.748 min, and 4.729 min for concentrations of 0.1 mg/ml, 0.08 mg/ml, 0.06 mg/ml, and 0.04 mg/ml, respectively. However, the test samples consisting of liver, ovary, and muscle extracts did not exhibit any characteristic peak indicating the presence of TTX in both seasons. Furthermore, bioassays conducted on the test samples did not show any toxicity symptoms, while standard test mice exhibited characteristic symptoms of TTX poisoning. Histopathological examinations revealed congestion, necrosis, degeneration, and hemorrhages in the liver, heart, and kidney tissues of the standard sample, but no changes were observed in the test sample. Based on the findings, it was concluded that Lagocephalus inermis, as exploited along the Karnataka coast, is not toxic at present. However, considering that the bacteria responsible for the species’ toxicity may not be sufficiently present to cause toxicity at the current time, it is imperative for Indian food sanitation authorities to continuously monitor and assess the toxicity of pufferfish consumed in terms of food safety.

Item Type:	Article
Uncontrolled Keywords:	Indian coast; Mouse Bioassay; Neurotoxin; Puffer fish; TTX
Subjects:	Biochemistry Fishery Biology
Divisions:	CMFRI-Mangalore CMFRI-Kochi > Marine Capture > Finfish Fisheries Division Subject Area > CMFRI > CMFRI-Kochi > Marine Capture > Finfish Fisheries Division CMFRI-Kochi > Marine Capture > Finfish Fisheries Division Subject Area > CMFRI-Kochi > Marine Capture > Finfish Fisheries Division
Depositing User:	Arun Surendran
Date Deposited:	26 Jun 2023 11:14
Last Modified:	26 Jun 2023 11:16
URI:	http://eprints.cmfri.org.in/id/eprint/17174

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