Cryopreservation of spermatophores of the marine shrimp, Penaeus indicus H. Milne Edwards

Diwan, A D and Joseph, Shoji (1999) Cryopreservation of spermatophores of the marine shrimp, Penaeus indicus H. Milne Edwards. Indian Journal of Fisheries, 46 (2). pp. 159-166.

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Abstract

Attempts on cryopreservation of spermatozoa have been made in penaeid shrimp, Penaeus indicus. Viable spermatozoa could be preserved successfully for a period of 60 days at -35°C and -196°C. Among several cryoprotectants used, a combination of DMSO (5 %) and glycerol (5 %), DMSO (5 %) and trehalose (0.25M) showed best viability rate (75 to 80 %) in freeze, thawed spermatozoa. Activational changes in spermatozoa during acrosome reaction after induction with egg water have also been recorded. The importance of cryogenic storage of spermatozoa is discussed.

Item Type: Article
Uncontrolled Keywords: Marine shrimp; Penaeus indicus; Cryoperservation; spermatophores
Subjects: Fish and Fisheries > Fish physiology
Crustacean Fisheries > Prawn and Prawn fisheries
Divisions: CMFRI-Cochin > Physiology and Nutrition Pathology
Subject Areas > CMFRI Brochures > CMFRI-Cochin > Physiology and Nutrition Pathology
Depositing User: Dr. V Mohan
Date Deposited: 16 Mar 2010 05:49
Last Modified: 09 Sep 2015 15:05
URI: http://eprints.cmfri.org.in/id/eprint/160

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