Diwan, A D and Joseph, Shoji (1999) Cryopreservation of spermatophores of the marine shrimp, Penaeus indicus H. Milne Edwards. Indian Journal of Fisheries, 46 (2). pp. 159-166.
Attempts on cryopreservation of spermatozoa have been made in penaeid shrimp, Penaeus indicus. Viable spermatozoa could be preserved successfully for a period of 60 days at -35°C and -196°C. Among several cryoprotectants used, a combination of DMSO (5 %) and glycerol (5 %), DMSO (5 %) and trehalose (0.25M) showed best viability rate (75 to 80 %) in freeze, thawed spermatozoa. Activational changes in spermatozoa during acrosome reaction after induction with egg water have also been recorded. The importance of cryogenic storage of spermatozoa is discussed.
|Uncontrolled Keywords:||Marine shrimp; Penaeus indicus; Cryoperservation; spermatophores|
|Subjects:||Fish and Fisheries > Fish physiology
Crustacean Fisheries > Prawn and Prawn fisheries
|Divisions:||CMFRI-Cochin > Physiology and Nutrition Pathology|
|Depositing User:||Dr. V Mohan|
|Date Deposited:||16 Mar 2010 05:49|
|Last Modified:||09 Sep 2015 15:05|
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