Jayasankar, P (2018) e DNA - A New Concept in Fisheries Research In: ICAR Sponsored Winter School on Recent Advances in Fishery Biology Techniques for Biodiversity Evaluation and Conservation, 1-21 December 2018, Kochi. [Teaching Resource]
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5-Winter School on Recent Advances in Fishery Biology Techniques for Biodiversity Evaluation and Conservation_2018_Jayasankar P.pdf Download (251kB) | Preview |
Abstract
Environmental DNA (eDNA) is defined as the genetic material obtained from a water sample containing no distinguishing signs of source macro-organisms. The method utilizes DNA which is continuously excreted by organisms into the surrounding environment through mucus, gametes, faeces, blood and other cells, and captures, analyses and obtains the nucleotide sequence of this DNA based on an environmental sample. eDNA analysis has emerged as a potentially powerful tool to access aquatic community structures. The inherent drawbacks in traditional approaches to monitor fish biomass/abundance in exploited waters can be overcome by employing eDNA techniques. Analysis of this eDNA can give us information on the organisms, their abundance and biomass through two approaches – eDNA barcoding and eDNA metabarcoding. In the former, specific species are targeted in samples using standard or quantitative PCR, and using traditional Sanger sequencing method. In the latter, the whole community is screened using multiple conserved primers and Next Gen Sequencing (NGS) is done. Studies suggest that eDNA metabarcoding outperforms traditional survey methods in terms of non-invasive sampling, sensitivity and cost incurred. There is now increased interest in using eDNA to supplement existing survey methods.
| Item Type: | Teaching Resource |
|---|---|
| Subjects: | Marine Fisheries |
| Divisions: | CMFRI-Kochi > Marine Capture > Pelagic Fisheries Division Subject Area > CMFRI > CMFRI-Kochi > Marine Capture > Pelagic Fisheries Division CMFRI-Kochi > Marine Capture > Pelagic Fisheries Division Subject Area > CMFRI-Kochi > Marine Capture > Pelagic Fisheries Division |
| Depositing User: | Arun Surendran |
| Date Deposited: | 04 Jan 2019 06:26 |
| Last Modified: | 15 Feb 2019 04:53 |
| URI: | http://eprints.cmfri.org.in/id/eprint/13309 |
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