Growth performance of Spirulina (Arthrospira) platensis in a low cost medium: An assessment

Ranjith, L and Shukla, S P and Vennila, A and Purushothaman, C S (2013) Growth performance of Spirulina (Arthrospira) platensis in a low cost medium: An assessment. Acta Biologica Indica, 2 (1). pp. 335-342.

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    Abstract

    The unialgal culture of Spirulina platensis was sub-cultured in Zarrouk’s medium under photoautotrophic conditions. Initially, indoor batch cultivation was carried out for a week in four different types of cultivation media viz., Zarrouk’s, Modified Zarrouk’s, prescribed Nallayam Research Centre (NRC), and Modified NRC. In modified medium, urea and phosphoric acid of NRC medium were replaced with sodium nitrate and dipotassium hydrogen phosphate (anhydrous) and concentration of ferrous sulphate heptahydrate was reduced. The batch and airlift indoor culture experiments were carried out with an illumination of 3500±100 lux, photoperiod of 12:12 hour light and dark periods and temperature of 24±1°C. The specific growth rate value was 5.7 % higher in Zarrouk’s medium as compared to modified NRC medium. However, the cost of modified NRC medium was considerably lower than Zarrouk’s medium; therefore, modified NRC medium was selected for outdoor studies. The outdoor mass cultivation was done under natural conditions with the solar radiation reaching the surface of culture was between 2160 and 8450 lux and temperature ranged from 27 to 34°C. An assessment of the performance of growth in batch, airlift and FRP (Fiber Reinforced Polymer) tanks revealed that culture grown in airlift units showed best growth which was evident from higher specific growth rate and number of doublings per day. There was a 3.4-fold increase in cell density (in terms of turbidity at 750 nm) of the cultures in such units. The growth in outdoor FRP tanks was also comparable to the airlift cultures.

    Item Type: Article
    Uncontrolled Keywords: Spirulina platensis, indoor culture, outdoor culture, medium, growth
    Subjects: Algae
    Divisions: CMFRI-Tuticorin
    Depositing User: Arun Surendran
    Date Deposited: 24 Jun 2017 08:55
    Last Modified: 24 Jun 2017 08:55
    URI: http://eprints.cmfri.org.in/id/eprint/11956

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