Raja Swaminathan, T and Lakra, W S and Gopalakrishnan, A and Basheer, V S and Kushwaha, B and Sajeela, K A (2012) Development and characterization of a fibroblastic-like cell line from caudal fin of the red-line torpedo, Puntius denisonii (Day) (Teleostei: Cyprinidae). Aquaculture Research, 43. pp. 498-508.
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Abstract
A fibroblastic-like cell line was established from the ornamental ¢sh, red-line torpedo (Puntius denisonii). The red-line torpedo fin (RTF) cell line is being maintained in Leibovitz’s L-15 mediumsupplementedwith 10% fetal bovine serum (FBS) for over 1year at 28 1C on a continuous basis in normal atmosphere. The growth rate of RTF cells increased as the FBS proportion increased from 5% to 20% at 28 1C with optimum growth at the concentrations of 10% FBS. The morphology of RTF cell was predominantly fibroblastic like. Propagation of these cell lines was serum dependent, with a low plating efficiency (o15%). Karyotyping analysis of RTF cells at the 25th passage indicated that the modal chromosome number was 2n550. The cell line was cryopreserved in liquid nitrogen at �196 1C and could be recovered from storage after 6 months with good cell viability. Polymerase chain reaction amplification of a fragment of two mitochondrial genes, 16S rRNA and CO1, con¢rmed the identity of these cell lines with those reported from this animal species, confirming that the cell lines originated from P. denisonii. The bacterial extracellular products from Vibrio cholerae MTCC3904 and Aeromonas hydrophila were found to be toxic to RTF. The cell lines were not susceptible to viral nervous necrosis virus, a marine ¢sh virus.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Identification; female specific protein; seabass; Lates calcarifer |
| Subjects: | Aquaculture > Tropical fish |
| Divisions: | Contributors |
| Depositing User: | Arun Surendran |
| Date Deposited: | 26 May 2017 09:47 |
| Last Modified: | 26 May 2017 09:47 |
| URI: | http://eprints.cmfri.org.in/id/eprint/11855 |
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