Identification of Random Amplified Polymorphic DNA (RAPD) Markers in Endemic Yellow Catfish, Horabagrus brachysoma (Gunther 1864)

Abdul Muneer, P M and Gopalakrishnan, A and Basheer, V S and Lakra, W S (2008) Identification of Random Amplified Polymorphic DNA (RAPD) Markers in Endemic Yellow Catfish, Horabagrus brachysoma (Gunther 1864). Asian Fisheries Science, 21. pp. 293-304.

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    Abstract

    Random amplified polymorphic DNA (RAPD) analysis was applied to individuals of Horabagrus brachysoma sampled from three geographic locations of Western Ghat river systems in India. Of the thirty-two 10-mer RAPD primers screened initially, ten were chosen and used in a comparative analysis of H. brachysoma collected from Chalakkudy, Meenachil and Nethravathi river systems. A total of 124 RAPD fragments were amplified, out of which 49 (39.51%) were found to be shared by individuals of all three river systems. The remaining 75 fragments were found to be polymorphic (60.48%). This confirms the suitability of RAPD markers for the study of population genetic structure in yellow catfish stocks.

    Item Type: Article
    Uncontrolled Keywords: Horabagrus brachysoma; RAPD; Genetic variation; Microsatellites; PCR; Polymorphism
    Subjects: Demersal Fisheries > Catfish
    Fish and Fisheries > Fish Genetics
    Divisions: CMFRI-Kochi > Marine Biotechnology
    Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology
    CMFRI-Kochi > Marine Biotechnology
    Subject Area > CMFRI-Kochi > Marine Biotechnology
    Depositing User: Arun Surendran
    Date Deposited: 20 May 2017 08:09
    Last Modified: 20 May 2017 08:09
    URI: http://eprints.cmfri.org.in/id/eprint/11784

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