Effect of cryoprotectants on the morphology of fish spermatozoa.

Bhavani, C. N (2002) Effect of cryoprotectants on the morphology of fish spermatozoa. Masters thesis, Central Institute of Fisheries Education.

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    Abstract

    A study was carried out to identify the best cryoprotectant for the cryopreservation of spermatozoa of Liza parsia, a brackish / marine fish of great culture potential. Various cryoprotectants alone and in combinations, at different concentrations have been tried. Morphology of spermatozoa before and after cryopreservation studied by transmission electron microscopy (TEM). A combination of 5% DMSO and 5% glycerol in V2E extender accorded better protection than 10% DMSO alone. The toxic effects of high concentrations of DMSO can be minimised by combining with glycerol.

    Item Type: Thesis (Masters)
    Uncontrolled Keywords: Cryoprotectants; Morphology; Fish Spermatozoa
    Subjects: Dissertations
    Fish and Fisheries > Fish breeding
    Divisions: CMFRI-Kochi > Mariculture
    Subject Area > CMFRI > CMFRI-Kochi > Mariculture
    CMFRI-Kochi > Mariculture
    Subject Area > CMFRI-Kochi > Mariculture
    Depositing User: Mr Rakesh
    Date Deposited: 05 Sep 2016 09:34
    Last Modified: 06 Oct 2016 10:49
    URI: http://eprints.cmfri.org.in/id/eprint/11037

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