Molecular phylogeny of commercially important lobster species from Indian coast inferred from mitochondrial and nuclear DNA sequences

Jeena, N S and Gopalakrishnan, A and Radhakrishnan, E V and Kizhakudan, Joe K and Basheer, V S and Asokan, P K and Jena, J K (2015) Molecular phylogeny of commercially important lobster species from Indian coast inferred from mitochondrial and nuclear DNA sequences. Mitochondrial DNA. pp. 1-10.

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    Abstract

    Lobsters constitute low-volume high-value crustacean fishery resource along Indian coast. For the conservation and management of this declining resource, accurate identification of species and larvae is essential. The objectives of this work were to generate species-specific molecular signatures of 11 commercially important species of lobsters of families Palinuridae and Scyllaridae and to reconstruct a phylogeny to clarify the evolutionary relationships among genera and species included in this study. Partial sequences were generated for all the candidate species from sampling sites along the Indian coast using markers like Cytochrome oxidase I (COI), 16SrRNA, 12SrRNA, and 18SrRNA genes, and analyzed. The genetic identities of widely distributed Thenus species along the Indian coast to be Thenus unimaculatus and the sub-species of Panulirus homarus to be P. homarus homarus were confirmed. Phylogeny reconstruction using the individual gene and concatenated mtDNA data set were carried out. The overall results suggested independent monophyly of Scyllaridae and Stridentes of Palinuridae. The interspecific divergence was found to be highest for the 12SrRNA compared with other genes. Significant incongruence between mtDNA and nuclear 18SrRNA gene tree topologies was observed. The results hinted an earlier origin for Palinuridae compared with Scyllaridae. The DNA sequence data generated from this study will aid in the correct identification of lobster larvae and will find application in research related to larval transport and distribution.

    Item Type: Article
    Uncontrolled Keywords: Mitochondrial markers; nuclear markers; lobsters
    Subjects: Fish Biotechnology
    Crustacean Fisheries > Lobsters
    Divisions: CMFRI-Kochi > Fishery Extension
    Subject Area > CMFRI > CMFRI-Kochi > Fishery Extension
    CMFRI-Kochi > Fishery Extension
    Subject Area > CMFRI-Kochi > Fishery Extension
    Depositing User: Arun Surendran
    Date Deposited: 17 Jun 2015 03:33
    Last Modified: 09 Sep 2015 16:01
    URI: http://eprints.cmfri.org.in/id/eprint/10401

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