Molecular cloning, characterization and expression analysis of cytoplasmic Cu/Zn-superoxid dismutase (SOD) from pearl oyster Pinctada fucata

Anju, A and Joseph, Jeswin and Thomas, P C and Paulton, M P and Vijayan, K K (2013) Molecular cloning, characterization and expression analysis of cytoplasmic Cu/Zn-superoxid dismutase (SOD) from pearl oyster Pinctada fucata. Fish & Shellfish Immunology, 34. pp. 946-950.

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Abstract

Because of its capacity to rapidly convert superoxide to hydrogen peroxide, superoxide dismutase (SOD) is crucial in both intracellular signalling and regulation of oxidative stress. In this paper we report the cloning of a Cu/Zn SOD (designated as pfSOD) from the pearl oyster (Pinctada fucata) using rapid amplification of cDNA ends (RACE) PCR. The full-length cDNA of this Cu/Zn SOD contains an open reading frame (ORF) of 471 bp coding for 156 amino acids. No signal peptide was identified at the N-terminal amino acid sequence of Cu/Zn SOD indicating that this pfSOD encodes a cytoplasmic Cu/Zn SOD. This is supported by the presence of conserved amino acids required for binding copper and zinc. Semi-quantitative analysis in adult tissues showed that the pfSOD mRNA was abundantly expressed in haemocytes and gill and scarcely expressed in other tissues tested. After challenge with lipopolysaccharide (LPS), expression of pfSOD mRNA in haemocytes was increased, reaching the highest level at 8 h, then dropping to basal levels at 36 h. These results suggest that Cu/Zn SOD might be used as a bioindicator of the aquatic environmental pollution and cellular stress in pearl oyster.

Item Type: Article
Uncontrolled Keywords: Cu/Zn SOD; Pinctada fucata; cDNA cloning; Phylogenetic analysis; gene expression
Subjects: Molluscan Fisheries > Pearl oyster
Fish Genetics
Divisions: CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division
Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division
CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division
Subject Area > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division
Depositing User: Arun Surendran
Date Deposited: 16 Jul 2013 10:20
Last Modified: 09 Sep 2015 15:55
URI: http://eprints.cmfri.org.in/id/eprint/9354

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