Diwan, A D and Joseph, Shoji (1998) Cryopreservation of spermatophores of the marine shrimp Penaeus indicus H. Milne Edwards. In: Fish Genetics and Biodiversity Conservation. Nature Conservators, Mussafarnagar, pp. 243-251.
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Abstract
Attempts on cryopreservation of spermatozoa have been made in penaeid shrimp Penaeus indicus. It was observed that viable spermatozoa could bepreserved successfully for a period of60 days at -35"C and -196"C temperature. Among several cryoprotectantsused a combination of DMSO (5%) + glycerol (5%) and DMSO (5%) + trehalose (0.25 M) showed best viability rate (75 to 80%) in freeze thawed spermatoza. The activational changes in spermatozoa during acrosome reaction after induction with anegg water have also been mentioned. The importance of cryogenic storage of spermatozoa has been discussed.
| Item Type: | Book Section |
|---|---|
| Uncontrolled Keywords: | Cryopreservation; spermatophores; marine shrimp; Penaeus indicus |
| Subjects: | Crustacean Fisheries > Prawn and Prawn fisheries |
| Divisions: | CMFRI-Kochi > Marine Capture > Crustacean Fisheries Division Subject Area > CMFRI > CMFRI-Kochi > Marine Capture > Crustacean Fisheries Division CMFRI-Kochi > Marine Capture > Crustacean Fisheries Division Subject Area > CMFRI-Kochi > Marine Capture > Crustacean Fisheries Division |
| Depositing User: | Arun Surendran |
| Date Deposited: | 05 Mar 2011 12:05 |
| Last Modified: | 09 Sep 2015 15:46 |
| URI: | http://eprints.cmfri.org.in/id/eprint/7896 |
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