Thomas, P C (2002) PCR based diagnostic techniques - Winter school on recent advances in diagnosis and management of diseases in mariculture, 7th to 27th November 2002, Course Manual. [Teaching Resource]
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Abstract
Use of PCR assisted diagnosis has many advantages over the conventional methods. Since PCR can amplify even a single strand of DNA into millions of copies within hours, even a pathogen collected from the infected fish could be used directly for the identification. Thus, considerable time can be saved. Two of the PCR based techniques merits special mention lor their use in rapid detection of microbial pathogens.. They are the DNA fingerprinting based on arbitrarily primed PCR (AP PCR) and amplification of species specific genes of the pathogens using specific primers flanking these genes.
| Item Type: | Teaching Resource |
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| Uncontrolled Keywords: | PCR diagnostic techniques; Winter school; diagnosis; management of diseases; mariculture; Course Manual |
| Subjects: | Aquaculture > Mariculture Aquaculture Fish Diseases |
| Divisions: | CMFRI-Kochi > Mariculture Division Subject Area > CMFRI > CMFRI-Kochi > Mariculture Division CMFRI-Kochi > Mariculture Division Subject Area > CMFRI-Kochi > Mariculture Division |
| Depositing User: | Mr. Arun Surendran |
| Date Deposited: | 25 Oct 2010 09:36 |
| Last Modified: | 09 Sep 2015 15:32 |
| URI: | http://eprints.cmfri.org.in/id/eprint/5364 |
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