PCR based diagnostic techniques - Winter school on recent advances in diagnosis and management of diseases in mariculture, 7th to 27th November 2002, Course Manual

Thomas, P C (2002) PCR based diagnostic techniques - Winter school on recent advances in diagnosis and management of diseases in mariculture, 7th to 27th November 2002, Course Manual. [Teaching Resource]

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Abstract

Use of PCR assisted diagnosis has many advantages over the conventional methods. Since PCR can amplify even a single strand of DNA into millions of copies within hours, even a pathogen collected from the infected fish could be used directly for the identification. Thus, considerable time can be saved. Two of the PCR based techniques merits special mention lor their use in rapid detection of microbial pathogens.. They are the DNA fingerprinting based on arbitrarily primed PCR (AP PCR) and amplification of species specific genes of the pathogens using specific primers flanking these genes.

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