Chromatographic separation of sugars

Saravanan, T S and Arumugam, M (1981) Chromatographic separation of sugars. CMFRI Special Publication (7). pp. 117-119.

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Abstract

The principle of chromatography involves separation of a mixture on the basis of specific differences in physical and chemical properties, which result from the structural differences of the chemically related groups of compounds which are under investigation. They therefore have differential affinity for both the mobile and stationary phases of the chromatographic systems. This chromatographic separation is the resultant of propelling (mobile phase) and retarding forces (stationary phase). The stationary phase in strict sense includes the medium (paper) together with the polar solvent (water). The mobile phase or propelling force includes both polar and non-polar solvent. The separation is brought about by continuous partition between the mobile phase (solvent flowing along the paper) and the water held in the paper and paper per se. Paper together with water acts as an adsorbent; it has a strong affinity for polar molecules which are held by hydrogen bonding and vander Waals' forces (Smith & Seakins, 1976).

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