Triploidy induction and confirmation in the edible oyster Crassosfrea madrasensis

Thomas, P C and Mallia, Jyothi V and Muthiah, P (2004) Triploidy induction and confirmation in the edible oyster Crassosfrea madrasensis. Journal of the Marine Biological Association of India, 46 (2). pp. 224-228.

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Abstract

This paper presents the protocols standardized for producing triploid edible oyster, Crassostrea madrasensis by retention of I1 polar body (MI1 triploids), using physical (heat and cold shocks) and chemical agents (Cytochalasin B and 6-Dimethylaminopurine), as well as the protocol for preparation of metaphase spreads for cytological confirmation of triploidy and estimation of its percentage. Among the four inducing agents tried, 6-Dimethylaminopurine yielded highest percentage of triploids (66.6 in day old and 61.82 in "D" stage larvae).

Item Type:	Article
Uncontrolled Keywords:	Triploidy induction; edible oyster; Crassosfrea madrasensis
Subjects:	Molluscan Fisheries > Edible oyster
Divisions:	CMFRI-Kochi > Physiology and Nutrition Pathology Subject Area > CMFRI > CMFRI-Kochi > Physiology and Nutrition Pathology CMFRI-Kochi > Physiology and Nutrition Pathology Subject Area > CMFRI-Kochi > Physiology and Nutrition Pathology
Depositing User:	Users 5 not found.
Date Deposited:	05 Aug 2010 12:25
Last Modified:	09 Sep 2015 15:15
URI:	http://eprints.cmfri.org.in/id/eprint/2035

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