Sulfated glycosaminoglycan CIP-3 from edible marine octopus Cistopus indicus: A promising bioactive with anti-hyperglycemic effects in 3T3-L1 adipocytes for functional food applications

Silpa, K P and Chakraborty, Kajal (2025) Sulfated glycosaminoglycan CIP-3 from edible marine octopus Cistopus indicus: A promising bioactive with anti-hyperglycemic effects in 3T3-L1 adipocytes for functional food applications. Food Bioscience, 66. pp. 1-11. ISSN 2212-4292

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Abstract

Marine cephalopod mollusks, particularly octopuses, are recognized as economically important seafood sources for their culinary delicacies and rich nutritional profiles. Sulfated glycosaminoglycans, a major component of these tissues, are widely acknowledged for their ability to reduce hyperglycemia, enhance glucose metabolism, and mitigate complications associated with type-2 diabetes. In this direction, a sulfated heteropolysaccharide (CIP-3), characterized as [→4)-β-GlcAp-(1 → 4)-3-O-SO3-β-GlcNAcp-(1→], with (1 → 6)-3,6-di-O-SO3-α-Manp linked to β-GlcNAcp, extracted from the edible tissues of the marine octopus Cistopus indicus (Rapp, 1835), exhibited promising anti-hyperglycemic activity in 3T3-L1 adipocytes. In vitro cell line analysis demonstrated that CIP-3 polysaccharide (25 μg mL−1) significantly enhanced glucose uptake in 3T3-L1 adipocyte cells by 39%, compared to untreated cells (⁓1%), highlighting its potential as an anti-hyperglycemic agent. Further, CIP-3 demonstrated significant anti-adipogenic activity by inhibiting intracytoplasmic triglyceride accumulation by ⁓52% compared to the negative control adipogenesis medium. In addition, CIP-3 significantly reduced dipeptidyl peptidase-4 (DPP-4) concentration (⁓75%) and downregulated DPP-4 enzyme activity by 65%, along with a further 63-fold increase in insulin secretion (3.15 μIU/mL) in RIN-5F cell lines. The presence of electronegative hydroxyl/carboxylic acid moieties, the C-3 and C-6 specific sulfation pattern, along with (1 → 4)/(1 → 6) glycosidic linkages, might be responsible for the promising anti-hyperglycemic abilities of CIP-3. Thus, the marine-derived CIP-3 polysaccharide, with potential attenuation activity against hyperglycemic disorders, might be anticipated as a prospective bioactive lead for functional food applications in managing type-2 diabetes.

Item Type:	Article
Uncontrolled Keywords:	Marine octopus; Cistopus indicus; Sulfated glycosaminoglycan; Anti-hyperglycemic 3T3-L1 adipocyte cells
Subjects:	Biochemistry > Bioprospecting Biochemistry > Bioactive compounds Molluscan Fisheries > Cephalopods Fish Biotechnology Molluscan Fisheries
Divisions:	CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division Subject Area > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division
Depositing User:	Arun Surendran
Date Deposited:	11 Mar 2025 05:30
Last Modified:	11 Mar 2025 05:30
URI:	http://eprints.cmfri.org.in/id/eprint/19376

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