Analytical instrumentation – working principles and applications in marine environment

Ratheesh Kumar, R (2024) Analytical instrumentation – working principles and applications in marine environment. In: Course Manual on "Know your Marine Biodiversity & Environment MarBiE 3" Water and Sediment Analytical Techniques with Special Reference to Marine Life. CMFRI Training Manual Series No.45/2024 . ICAR- Central Marine Fisheries Research Institute, Kochi, pp. 70-72.

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Abstract

UV Visible spectrophotometer measures the response of a sample to ultraviolet and visible range of electromagnetic radiations. It operates by passing a beam of light through a sample and measuring the intensity of light reaching the detector. A spectrophotometer consists of two main components, namely a spectrometer for producing light of any selected wavelength, and a photometer for measuring the intensity of light. The instruments are designed in such a way that that liquid in a cuvette can be placed between the spectrometer beam and the photometer. The amount of light passing through the cuvette is measured by the photometer. The signal changes as the amount of light absorbed by the liquid changes. According to Beer's law, when light of a specific wavelength passes through a solution there is usually a quantitative relationship between the solute concentration and the intensity of the transmitted light. The O.D. value recorded is directly proportional to the concentration of the coloured compound. Most spectrophotometers have a scale that reads both in O.D. (absorbance) units, which is a logarithmic scale, and in % transmittance, which is an arithmetic scale. As suggested by the above relationships, the absorbance scale is the most useful for colorimetric assays. The applications of spectrophotometer include water quality assessment and other quantitative and qualitative analysis, enzyme assays, turbidimetry, nephelometry etc

Item Type:	Book Section
Uncontrolled Keywords:	marine environment
Subjects:	Marine Environment
Divisions:	CMFRI-Kochi > Marine Biodiversity, Environment and Management Division Subject Area > CMFRI > CMFRI-Kochi > Marine Biodiversity, Environment and Management Division CMFRI-Kochi > Marine Biodiversity, Environment and Management Division Subject Area > CMFRI-Kochi > Marine Biodiversity, Environment and Management Division
Depositing User:	Arun Surendran
Date Deposited:	13 Dec 2024 09:02
Last Modified:	13 Dec 2024 09:02
URI:	http://eprints.cmfri.org.in/id/eprint/19149

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