Establishment of immortal marine fish cell lines as in vitro tools for advancing research in environmental monitoring, biotechnology and biodiversity conservation

Sobhana, K S and George, Githa Ann and Sunny, Sheetal Mary and Poulose, Priyanka and Vijayan, Swathy and Joshi, K K and Gopalakrishnan, A and George, Grinson (2024) Establishment of immortal marine fish cell lines as in vitro tools for advancing research in environmental monitoring, biotechnology and biodiversity conservation. Marine Fisheries Information Service No.260. pp. 11-17. ISSN 0254-380X

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Abstract

Immortalized cell lines are invaluable to researchers as they can proliferate indefinitely, allowing them to be cultured across multiple generations. Unlike primary cells with a limited lifespan, immortal cell lines (also called as continuous cell lines) bypass challenges such as ethical concerns, difficulty in extraction, limited passage ability, and inconsistent results due to variations in cell sources. Most cells in laboratory conditions face the Hayflick limit, where telomeres shorten with each division, leading to senescence. Immortalized cell lines overcome these limitations, enabling stable, long-term studies while eliminating the need for repeated cell isolation and cultivation, thus saving both time and resources in research. Continuous cell lines can proliferate indefinitely in vitro, providing a sustainable and reproducible system for a wide range of research purposes. These cell lines offer a unique platform for investigating organisms at the cellular and molecular levels, providing insights that are not always possible with whole-organism models. It replicates the cellular and genetic homogeneity of the host, while minimising the variability inherent with in vivo systems. Consequently, there has been a growing focus on developing new cell lines to support a broader array of biological investigations.

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