Anti-coagulant effects of α-(1 → 3)/(1 → 4) linked sulfated galactofucan from brown macroalga Sargassum plagiophyllum (C. Agardh) in human umbilical vein endothelial cells

George, Ans Mariya and Chakraborty, Kajal and Silpa, K P and Bose, Chaithanya and Dhara, Subhajit (2024) Anti-coagulant effects of α-(1 → 3)/(1 → 4) linked sulfated galactofucan from brown macroalga Sargassum plagiophyllum (C. Agardh) in human umbilical vein endothelial cells. Food Bioscience, 62. pp. 1-14. ISSN 2212-4306

Text Food Bioscience_2024_Kajal Chakraborty.pdf Restricted to Registered users only Download (6MB) | Request a copy

Abstract

Polysaccharides from brown macroalgae have been recognized as a nutraceutical source due to their combined nutritional and medicinal benefits. The sulfated derivatives of these polysaccharides are recognized for their potential as anticoagulants. Brown seaweeds, particularly those belonging to the family Sargassaceae, are valuable resources of sulfated polysaccharides. This study characterized the anticoagulant potential of a sulfated galactofucan (SP2) from Sargassum plagiophyllum, composed of α-(1 → 3)/(1 → 4) Fucp monomers linked to α-galactose and its interactions with key coagulation factors involved in the coagulation cascade. SP2, at concentrations ranging from 6.25 to 25 μg/mL, exhibited significant anticoagulant activity by prolonging activated partial thromboplastin time to 147.10s, surpassing the effect of heparin (124.44s). Additionally, SP2 showed comparable efficacy in the prothrombin time assay conducted on human umbilical vein endothelial cells. At 25 μg/mL, SP2 treatment led to an approximate 84% reduction in phospho-MARCKS expression and a seven-fold inhibition of coagulation factor Xa, highlighting its significant anticoagulant potential. At 25 μg/mL, SP2 reduced thrombin-induced intracellular Ca2⁺ mobilization by 48%, inhibited fibrin polymerization by 85%, and led to a 74% decrease in total thrombin production compared to the negative control. Additionally, SP2 treatment resulted in a 64% reduction in ADP-induced platelet aggregation, further highlighting its potent anticoagulant effects. The anticoagulant effects of SP2 might be attributed to the 2-O/3-O sulfation pattern, which facilitates its binding to positively-charged regions on coagulation proteins, thereby preventing clot formation. This multi-target approach of SP2 in the coagulation cascade clearly emphasizes its potential for preventing abnormal blood clotting and managing thrombotic disorders.

Actions (login required)

View Item