Extraction of Otoliths -Practical guidelines

Sangeetha, A T and Vishnupriya, K M and Nair, Rekha J (2025) Extraction of Otoliths -Practical guidelines. ICAR Short Term Training Manual on Integrated Taxonomic Techniques for Marine Biodiversity Conservation. pp. 134-136.

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Abstract

Otoliths are small calcium carbonate structures found in the inner ear of fish and are used for age determination. Place the fish on a clean dissecting tray with its dorsal (top) side facing upward. If large, secure the fish to prevent slipping. Feel for the bony part of the head just behind the eyes; this is where the otoliths are located. Use a sharp knife or scalpel to cut through the top of the skull. Make a small incision from just behind the eyes towards the middle of the head. Be careful not to damage the otoliths. Carefully lift or remove the skull bone to expose the brain. The otoliths are located beneath the brain in the inner ear chambers. Use forceps or tweezers to extract the otoliths gently. There are typically three pairs of otoliths: the sagittae (largest), lapilli, and asterisci. The sagittae otoliths are commonly used for aging studies. Rinse with clean water or ethanol to remove any tissue or debris. Airdry or store in a labeled vial for further analysis.

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