Marine Macroalga-associated Bacillus amyloliquefaciens as Prospective Probiotic

Vinaya, K K and Chakraborty, Kajal (2021) Marine Macroalga-associated Bacillus amyloliquefaciens as Prospective Probiotic. Journal of Aquatic Food Product Technology, 30 (9). pp. 1078-1089. ISSN 1547-0636

Text Journal of Aquatic Food Product Technology_2021_Kajal Chakraborty.pdf Restricted to Registered users only Download (3MB) | Please mail the copy request to cmfrilibrary@gmail.com

Abstract

Heterotrophic Bacillus amyloliquefaciens (MTCC 12716) isolated from the red alga Hypnea valentiae exhibited promising antibacterial activity (minimum inhibitory concentration of ≤1.0 µg/mL) against clinically relevant pathogens. The bacterium displayed bile salt and acid tolerance up to 4 h in a simulated gastric fluid, along with a prospective antioxidant effect. This non-hemolytic bacterium was found to be sensitive to a panel of antibiotics and did not amplify the enterotoxin-specific genes. In order to analyze the possibilities of scaling up, we have optimized the growth of B. amyloliquefaciens by response surface methodology to realize maximum production of anti-infective agents in the fermentation medium and observed an increment of about 85% antibacterial activity against gastrointestinal pathogens. As probiotic treatment is useful due to its ability to recreate the natural intestinal microflora, this heterotrophic Bacillus could be contemplated to offer a prospective probiotic. Further studies are needed to assess its utility for commercial application.

Item Type:	Article
Uncontrolled Keywords:	Macroalga-associated Bacillus amyloliquefaciens; probiotic effects; gastrointestinal pathogens; antibacterial activity; enterotoxin specific genes
Subjects:	Algae > Bacteria Biochemistry Algae
Divisions:	CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division Subject Area > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division
Depositing User:	Arun Surendran
Date Deposited:	25 Jan 2023 06:18
Last Modified:	25 Jan 2023 06:18
URI:	http://eprints.cmfri.org.in/id/eprint/16644

Actions (login required)

View Item