Analysis of nonribosomal peptide synthetase genes in haemolymph microbes of marine crabs

Mary Benny, Akhitha and Sumithra, T G and Reshma, K J and Nair, V Anusree and Bindu Raj, Neethu and Amala, P V and Ratheesh Kumar, R and Soni, K B and Sanil, N K (2021) Analysis of nonribosomal peptide synthetase genes in haemolymph microbes of marine crabs. Archives of Microbiology, 203. pp. 1251-1258.

Text Archives of Microbiology_2021_Sumithra TG.pdf Restricted to Registered users only Download (1MB) | Request a copy

Abstract

Nonribosomal peptide synthetases (NRPS) are multi-domain enzymes that have innumerably beneficial health applications. Realizing the significance of marine microorganisms in search for NRPS sequences, study was conducted for analysis of NRPS gene sequences of marine crab haemolymph bacteria for the first time. Strains belonging to five different species were found to have NRPS genes. The study generated NRPS sequences from four bacterial species, for which NRPS gene information was not available earlier. Two new putative adenylation domain signatures were identified from phylum Firmicutes. In silico analysis of amino acid sequences from four species showed less identity (42–50%) to the characterized NRPS compounds that integrate serine residue in active site, suggesting the novelty or uncharacterized nature. Altogether, the study warrants future research exploiting marine crab haemolymph bacteria, an unexplored niche of microbial genetic wealth to discover microbial novel NRPS genes and natural products using emerging tools and technologies.

Actions (login required)

View Item