Polygalactan from bivalve Crassostrea madrasensis attenuates nuclear factor- κB activation and cytokine production in lipopolysaccharide-activated macrophage

Chakraborty, Kajal and Krishnan, Soumya and Joy, Minju (2020) Polygalactan from bivalve Crassostrea madrasensis attenuates nuclear factor- κB activation and cytokine production in lipopolysaccharide-activated macrophage. Carbohydrate Polymers, 249. pp. 1-11.

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Abstract

A polygalactosamino-glucopyranosyl fucopyranose →4)-β-GlcAp{(3→1)-α-Fucp}-β-GalNAcp-(4,6-SO3−)-(1→ isolated from the bivalve Crassostrea madrasensis exhibited prospective anti-inflammatory activity against cyclooxygenase-2 and 5-lipoxygenase (IC50 < 50 μg mL−1) on lipopolysaccharide-induced macrophages. The polygalactan attenuated inducible nitric oxide synthase (IC50 65.7 μg mL−1) in lipopolysaccharide-prompted inflammation leading to the reduction of pro-inflammatory cytokine nitric oxide (236.2 μg mL−1 lysate), nuclear factor-κB, tumor necrosis factor-α, and interleukins (0.19−0.22 units mg−1 protein at 100 μg mL-1) by inhibiting cyclooxygenase-2. The polygalacatan suppressed the mRNA of nuclear factor-κB and cyclooxygenase-2 in lipopolysaccharide-induced macrophages. Western blot experiment revealed that the polygalactan attenuated the migration of nuclear factor-κB-p65 to the nucleus from cytoplasm, and suppressed the phosphorylation of α-subunit of κB inhibitor. Greater selectivity index of sulfated polygalactan (3.93) towards inducible cyclooxygenase-2 as compared with the anti-inflammatory agent ibuprofen (1.11), and the potential to inhibit nuclear factor-κB cascade to generate chemokine production manifested its utilization in the development of functional food attenuating inflammation-related disorders.

Item Type:	Article
Uncontrolled Keywords:	Crassostrea madrasensis; Polygalactosamino-glucopyranosyl; fucopyranose; Cyclooxygenase-2; Lipopolysaccharide-induced macrophages; Cytokines; Nuclear factor-κB
Subjects:	Biochemistry > Bioactive compounds Biochemistry Molluscan Fisheries > Bivalves Fish Biotechnology
Divisions:	CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division Subject Area > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division
Depositing User:	Arun Surendran
Date Deposited:	14 Aug 2020 06:26
Last Modified:	14 Aug 2020 06:26
URI:	http://eprints.cmfri.org.in/id/eprint/14471

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