Joseph, Dexy and Chakraborty, Kajal (2017) Enrichment of C20-22 Polyunsaturated Fatty Acids from Refined Liver Oil of Leafscale Gulper Shark, Centrophorus squamosus. Journal of Aquatic Food Product Technology, 26 (9). pp. 1042-1056.
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Journal of Aquatic Food Product Technology_2017_Kajal Chakraborty_Enrichment of C20-22 polyunsaturated fatty acids from refined liver oil of Leafscale Gulper Shark.pdf Download (1MB) | Preview |
Abstract
The C20-22 polyunsaturated fatty acids (C20-22 PUFAs), mainly eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3), were concentrated from the refined liver oil of deep sea leafscale gulper shark, Centrophorus squamosus, by sequential processes of winterization, urea complexation, and argentation chromatography. Winterization at 4°C using acetonitrile as solvent showed significant reduction of the total saturated fatty acid content (12%) with a concomitant increase of polyunsaturated fatty acids (36.3%). The urea complexation process significantly enriched the total polyunsaturated fatty acids (49.5%), with a reduction in saturated fatty acids (83.9%). Silica gel based argentation chromatography further concentrated the esters of C20-22 PUFA (>99 percent purity). Nuclear Magnetic Resonance spectroscopy was used as a chemical fingerprinting tool to study the enrichment of C20-22 PUFAs at various stages of the purification process. An increase in the proton signal intensity at the olefinic region (at δ 4–6) and a decrease in the aliphatic signals (at δ 0.5–2) showed that the process successfully enriched the C20-22 PUFAs.
| Item Type: | Article |
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| Uncontrolled Keywords: | C20-22 polyunsaturated fatty acids; deep sea leafscale gulper shark; Centrophorus squamosus; winterization; urea complexation; argentation chromatography |
| Subjects: | Biochemistry > Bioprospecting Biochemistry > Bioactive compounds Biochemistry Fish Biotechnology |
| Divisions: | CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division Subject Area > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division |
| Depositing User: | Arun Surendran |
| Date Deposited: | 13 May 2019 05:50 |
| Last Modified: | 13 May 2019 05:50 |
| URI: | http://eprints.cmfri.org.in/id/eprint/13648 |
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