In vitro Pearl Culture Techniques: A Biotechnological Approach

Suja, C P (2019) In vitro Pearl Culture Techniques: A Biotechnological Approach. CMFRI Special Publication (131). ICAR - Central Marine Fisheries Research Institute, Kochi.

CMFRI SP No.131_In vitro pearl culture technics_Eprints.pdf

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    This book deals with the set up of tissue culture laboratory, protocols for maintaining a laboratory and methods for different experiments for nacre layer formation to achieve in vitro pearl formation. Indeed, this study explains the mantle tissue culture techniques of different pearl producing molluscs, cell proliferation with pearl sac formation and crystal deposition which form the basis for in vitro pearl formation. The techniques and methodologies used for pearl sac formation are unique and achieved after several trials and errors. Studies with different species of pearl producing molluscs reveal that, all the species are capable of producing cells, pearl sac and nacre crystals by techniques used in this study. Moreover, results of this study revealed that the shell bead is the best material for crystal adhesion. Formation of nacre layers is proved by Raman spectroscopy, Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray Spectroscopy (EDS) in all the experimental shell beads. It is the first authenticated report of nacre layer formation over the shell bead in in vitro conditions with mantle cells of different pearl producing molluscs such as Pinctada fucata, Haliotis varia and Pteria avicular. This study is the stepping stone and needs further research to produce more lustrous pearls for commercialization.

    Item Type: Book
    Uncontrolled Keywords: In vitro Pearl Culture Techniques
    Subjects: Molluscan Fisheries > Pearl oyster
    CMFRI Publications > CMFRI Special Publications
    Divisions: CMFRI-Tuticorin
    Depositing User: Arun Surendran
    Date Deposited: 14 Feb 2019 10:41
    Last Modified: 16 Mar 2023 06:23

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