Antioxidative sulphated polygalactans from marine macroalgae as angiotensin-I converting enzyme inhibitors

Makkar, Fasina and Chakraborty, Kajal (2018) Antioxidative sulphated polygalactans from marine macroalgae as angiotensin-I converting enzyme inhibitors. Natural Product Research, 32 (17). pp. 2100-2106. ISSN 1478-6427 (Online)

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Abstract

Antioxidant and antihypertensive potential of the sulphated polygalactans isolated from the marine macroalgae Kappaphycus alvarezii and Gracilaria opuntia were assessed by utilising different in vitro systems. The galactans isolated from K. alvarezii possessed significantly greater antioxidative properties as determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH IC90 0.97 mg/mL) and 2,2′-azino-bis(3-ethylbenzothiazoline-6- sulphonic acid (ABTS.+ IC90 0.72 mg/mL) scavenging activities than those isolated from G. opuntia (DPPH IC90 1.2 mg/mL and ABTS 0.86 mg/mL). The sulphated polygalactan →4)-4-O-sulphonato-(2-O-methyl)-β-Dgalactopyranosyl-( 1→4)-3,6-anhydro-(2-O-methyl)-α-D-galactopyranan from K. alvarezii showed greater angiotensin-I-converting enzyme (ACE) inhibitory activity (IC50 0.02 μg/mL) than →3)-4-O-sulphonato-(6-Oacetyl)- β-D-galactopyranosyl-(1→4)-3,6-anhydro-(2-O-sulphonato)-α- D-galactopyranosyl-(1→3)-4-O-sulphonato-(6-O-acetyl)-β-D-xylosyl- (1→3)-4-O-sulphonato-(6-O-acetyl)-β-D-galactopyranosyl-(1→4)-3,6- anhydro-(2-O-sulphonato)-α-D-galactopyranan motif extracted from G. opuntia (IC50 0.70 μg/mL). Structure activity correlation studies displayed that the ACE inhibitory properties of titled polygalactans were directly proportional to their electronic properties and inversely with the steric and hydrophobic characteristics. Putative ACE inhibitory mechanism of action of sulphated galactans from marine macroalgae corroborated the structure bioactivity correlation analysis.

Item Type:	Article
Uncontrolled Keywords:	Sulphated polygalactan; antioxidant activity; angiotensin-I-converting enzyme; marine macroalgae
Subjects:	Biochemistry > Bioactive compounds Biochemistry Algae
Divisions:	CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division Subject Area > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division
Depositing User:	Arun Surendran
Date Deposited:	02 Aug 2018 04:15
Last Modified:	02 Aug 2018 04:15
URI:	http://eprints.cmfri.org.in/id/eprint/12982

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