Concentration of C20-22 n-3 polyunsaturated fatty acids from Sardinella longiceps and fatty acid stabilization

Chakraborty, Kajal and Joseph, Deepu and Joseph, Dexy (2016) Concentration of C20-22 n-3 polyunsaturated fatty acids from Sardinella longiceps and fatty acid stabilization. European Journal of Lipid Science and Technology, 118 (2). pp. 208-223.

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Abstract

Long chain n-3 polyunsaturated fatty acids from the refined oil from Sardinella longiceps were concentrated by low-temperature crystallization and urea complexation. Low-temperature crystallization (4°C) using acetone:ethylacetate (7:3, v/v) and urea complexation with urea-to-fatty acid ratio of 4:1 (w/w) afforded fatty acid concentrate (FAC) with greater than 60% C20–22 n-3 fatty acids, and significant increase in NMR olefinic proton integral (at d 5.2–5.6). Ethylacetate fractions of seaweeds, Kappaphycus alvarezii, Hypnea musciformis, and Jania rubens, which possessed greater antioxidant activities, were added to FAC individually, and in nine factorial combinations (0.5%, w/w). The oxidative stabilities of these treatments were compared with FAC added with BHT and a-tocopherol in an accelerated storage study. A synergistic effect of ethylacetate fractions from K. alvarezii, H. musciformis, and J. rubens used at 0.1:0.2:0.2 (%, w/w) in preventing the oxidative degradation of FAC was observed. No traces of aromatics (d 6–7) and aldehydes (d 9–10) were present in the 1H-NMR spectra of FAC added with seaweed extracts after the accelerated storage study, demonstrating that the seaweed extracts were able to prevent the formation of low molecular weight secondary oxidation products in the unsaturated system during storage.

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