Pramitha, V S and Lipton, A P and Thangaraj, M (2008) Evaluation of α- and β-glucosidase inhibitory properties of macro-algae using intestinal extracts of marine snail, Thais rudolphi (Lamarck, 1822). Indian Journal of Biotechnology, 7 (1). pp. 66-72.
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Abstract
Glucosidase-inhibitory activity of marine macro-algae, Ulva fasciata and Hypnea musciformis in different solvents was detected using enzyme-agar plate method. Eenzyme-agar plate containing α- or β-D-glucosidase was used in combination with substrate-agar plate containing p-nitro phenyl α- and β-D-glucopyranoside (PNPG). Discs impregnated with algal extracts placed on the enzyme agar, followed by pre-incubation up to 2.5 h at 25°C, resulted in the characteristic inhibitory circle around the inhibitor. Extracts of red algae, H. musciformis had higher glucosidase-inhibition than the extracts of U. fasciata. The intestine of Thais rudolphi contained both α- and β-glucosidase enzymes and their inhibition was comparable with the commercial enzymes, suggesting the possible alternate method for assessing the glucosidase-inhibitory activity of the marine macro-algae
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Glucosidases; Hypnea musciformis; macro-algal inhibitors; rapid assay; Thais rudolphi; Ulva fasciata |
| Subjects: | Molluscan Fisheries Fish Biotechnology > Bioactive compound |
| Divisions: | CMFRI-Vizhinjam |
| Depositing User: | Arun Surendran |
| Date Deposited: | 29 Jul 2014 04:55 |
| Last Modified: | 09 Sep 2015 15:59 |
| URI: | http://eprints.cmfri.org.in/id/eprint/10140 |
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