Differential responses of sexual and asexual Artemia to genotoxicity by a reference mutagen:Is the comet assay a reliable predictor of population level responses?

Sukumaran, Sandhya and Grant, Alastair (2013) Differential responses of sexual and asexual Artemia to genotoxicity by a reference mutagen:Is the comet assay a reliable predictor of population level responses? Ecotoxicology and EnvironmentalSafety, 91. pp. 110-116.

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Abstract

The impactofchronicgenotoxicitytonaturalpopulationsisalwaysquestionedduetotheir reproductivesurplus.WeusedacometassaytoquantifyprimaryDNAdamageafterexposuretoa referencemutagenethylmethanesulfonateintwospeciesofcrustaceanwithdifferentreproductive strategies(sexual Artemia franciscana and asexual Artemia parthenogenetica). Wethenassessedwhether this predictedindividualperformanceandpopulationgrowthrateoverthreegenerations. Artemia were exposedtodifferentchronicconcentrations(0.78mM,1.01mM,1.24mMand1.48mM)ofethyl methanesulfonatefrominstar1onwardsfor3h,24h,7days,14daysand21daysandpercentagetail DNA valueswereusedforcomparisonsbetweenspecies.ThepercentagetailDNAvaluesshowed consistentlyelevatedvaluesupto7daysandshowedareductionfrom14daysonwardsin A. franciscana. Whilstin A. parthenogenetica such areductionwasevidenton21daysassessment.The values ofpercentagetailDNAafter21dayswerecomparedwithpopulationlevelfitnessparameters, growth,survival,fecundityandpopulationgrowthratetoknowwhetherprimaryDNAdamageas measuredbycometassayisareliablebiomarker.SubstantialincreaseintailDNAvalueswasassociated with substantialreductionsinallthefitnessparametersintheparentalgenerationof A. franciscana and parental,F1andF2generationsof A. parthenogenetica. Socometresultsweremorepredictiveinasexual speciesovergenerations.Theseresultspointedtotheimportanceofpredictingbiomarkerresponses from multigenerationalconsequencesconsideringlifehistorytraitsandreproductivestrategiesin ecologicalriskassessments.

Item Type:	Article
Uncontrolled Keywords:	Artemia franciscana; Artemia parthenogenetica; Ethyl methanesulfonate; Genotoxicity; Comet assay; Fitness effects
Subjects:	Fish Genetics
Divisions:	CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division Subject Area > CMFRI > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division Subject Area > CMFRI-Kochi > Marine Biotechnology, Fish Nutrition and Health Division
Depositing User:	Arun Surendran
Date Deposited:	30 Oct 2013 09:42
Last Modified:	09 Sep 2015 15:56
URI:	http://eprints.cmfri.org.in/id/eprint/9638

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