Genetic characterization and protein profiling of green mussel (Perna viridis (Linnaeus, 1758)) and brown mussel (Perna indica Kuriakose and Nair, 1976)

Divya, P R (2008) Genetic characterization and protein profiling of green mussel (Perna viridis (Linnaeus, 1758)) and brown mussel (Perna indica Kuriakose and Nair, 1976). PhD thesis, Central Marine Fisheries Research Institute. Submitted to Central Institute of Fisheries Education.

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Abstract

The marine mussels of India are mainly of two species: Perna viridis [Linnaeus, 1758) (green mussel) and Perna indica Kuriakose and Nair (1976) [brown mussel). In addition to this, along the south west coast (Kollam coast f Kerala), a third type of mussel called parrot mussel has also been reported. The present study was undertaken for genetic characterisation and dentification of distinct stocks if any, within green as well as brown mussels, From both east and west coast, using shell morphometrics and molecular markers. Stock identification using shell morphometrics were attempted with Principal Component Analysis and Canonical Discriminant function Analysis, whereas, molecular methods used were protein electrophoresis (Native - Poly Acrylamide Gel Electrophoresis) and Randomly Amplified Polymorphic DNAPolymerase Chain Reaction (RAPD-PCR). The shell morphometrics as well as Native PAGE and RAPD-PCR profiles gave concordant results indicating high intraa species homogeneity and low level of genetic differentiation among populations of both green and brown mussels. Genetic characterisation using RAPD markers could bring out the genetic variability within both the populations. Elucidation of the genetic identity of parrot mussel as to whether it is a true hybrid of green and brown mussel, or only a colour variant of any of them was also done using RAPD, shell morphometrics, protein electrophoresis (native PAGE, SDS (Sodium Dodecyl Sulphate) PAGE and allozymes) and mitochondria1 (mt) DNA gene (Cytochrome oxidase 1) analysis. The study concluded that the parrot mussel is not a hybrid of the two, but only a colour variant of the brown mussel. An initial attempt to resolve the taxonomic ambiguity of P.indica that it is only a synonym of globally distributed Perna perna, as suggested by some of the previous workers has also been made in this study using COI regions of mt DNA. As P. indica revealed only 5% divergence from P, perna, it was concluded that P. indica need not be relegated as a synonym of P.perna

Item Type:Thesis (PhD)
Supervisor(s):Thomas, P C
Uncontrolled Keywords:Genetic characterization; protein profiling; green mussel ; Perna viridis; brown mussel; Perna indica
Subjects:Fish and Fisheries > Fish Genetics
Fish Biotechnology > Microbiology
Theses
Fish Biotechnology > Bioactive compound
Divisions:CMFRI-Cochin > Marine Biotechnology
ID Code:8912
Deposited By:Arun Surendran
Deposited On:09 Mar 2012 10:43
Last Modified:28 May 2012 15:04

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